VL6 Medium without Glucose, Animal Free Media Size:6 x 1kg

VL6 Medium without Glucose, Animal Free Media Size:6 x 1kgVL6 is a complex medium for cultivation of Dictyostelium discoideum based on Vegetable Peptone. Formedium Ltd have recognised the need for a range of meat free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C. VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source. Yeast Extract is a source of

9% Purity (TLC) One Spot IR Conform standard Solubility (5%

The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation

0005% Chloride (Cl) <0

5 α-Lactose 2

Tryptone 10 Yeast extract 5 Final pH 7

98 Agar 15 Final pH 7

49 Suspend 7

015 NH4 < 1

4 Folic acid 0

By omitting Folic acid and Riboflavine from the original YNB w/o Amino acids formulation the result is a very low fluorescence back ground medium

Suspend 56

Yeast Nitrogen Base without Amino Acids and without Ammonium Sulfate has the same formulation as Yeast Nitrogen Base without Amino Acids except that Ammonium Sulfate as a source of Nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources for example Potassium Nitrate